Isolation
of bone marrow stroma-derived adult stem cells and
the possibility for their application for tooth regeneration
Kazuhito Satomura, Eriko
Maeda, Reiko Tokuyama, Keiko Kudoh, Kazuhisa Taoka
Davaadorj Purevsren,
Yoshiko Yamamura
Department of Oral and
Maxillofacial Surgery, Institute of health Biosciences,
The
University of
We isolated mouse bone marrow stroma-derived adult stem
cells (MMSC-3) which could be induced for the differentiation not only into mesodermal cells, but also into ectodermal
and endodermal cells in vitro. In order to
investigate the possibility of MMSC-3 for the differentiation into odontogenic lineage, MMSC-3 was co-cultured with a rat
dental epithelial cell line in the presence or absence of various extracellular matrices such as EMD, Matrigel
or GFR Matrigel.
These investigaions revealed that 1) MMSC-3
was capable of differentiating into odontogenic cell lineage, 2) the odontogenic
differentiation was promoted by the odontogenic
epithelial cells.