Experimental Study of Antitumor
Effects of Bleomycin using Electrochemotherapy
on Human Uterine Squamous Cell Carcinoma.
Yoshihiko Sugita, Masato Jinno, Kouhei Takayama,
Yuuma Honda,
Katsutoshi Kubo, Emiko
Sato, Hatsuhiko Maeda
1) Department of Oral Pathology,
2) Research Institute of Advanced Oral Science,
The aim of
this study was determine the effects of electrochemotherapy
with bleomycin on human uterine squamous cell carcinoma. Caski cells were transplanted into the dorsal
subcutaneous tissue of nude mice. After
transplantation, the animals were randomly divided into four equal groups.
These groups received either no treatment; bleomycin
treatment withour electroporation;
electroporation without bleomycin
treatment; or bleomycin treatment with electroporation. These treatments performed four times of
0, 1, 7, 8 days, and the tumor volume were measured every other day for 14
days, and were investigated histologically. Bleomycin treatment with electroporation
markedly reduced the size of the tumor, whereas bleomycin
treatment or electroporation alone did not. These
results clearly indicate that the antitumor effect of
bleomycin was considerably potentiated
or enhanced by the administration of electroporation.