Nicotine increase the expression of carbonic anhydrase II in RAW 264.7 cell

Hideki Tanaka^*, Naoto Suzuki**^, ***, Masafumi Motohashi*^, ***,

Kichibee Otsuka**^, ***, Maeno Masao*^, ***

Departments of Oral Health Sciences*, Biochemistry**,

Division of Functional Morphology, Dental Research Center***,

Nihon University School of Dentistry

Several studies have indicated that tobacco smoking may be an important risk factor for the development and severity of inflammatory periodontal disease. We examined the effects of nicotine, which is a majour component of tobacco, on the expression of carbonic anhydrase II (CAII), cathepsin K, matrix metalloproteinase (MMP)-9 (MMP-9) , and α7 nicotine receptor in RAW264.7. In the presence of nicotine, the expression of CAII increased markedly , whereas the expression of MMP9, cathepsin K, and α7 nicotine receptor was not affected .　These results suggest that nicotine stimulates dissolution of inorganic in alveolar bone via increasing the production of CAII.