Immunohistochemical changes of
Runx2 and Msx2 in mouse periodontal tissues elicited by orthodontic force
Takehiro Watanabe*,**, Rina
Muraoka*,**, Takako Shimizu**, Keisuke Nakano*,***, Norimasa Okafuji*, Saburo Kurihara***, Kazuhiro Yamada*,**, Toshiyuki Kawakami*,***
Matsumoto Dental
University Institute for Oral Science***
Early changes of Runx2 and Msx2
expressions were examined by immunohistochemistry in mouse
periodontal ligament exposed to mechanical stress. Strong expressions of Runx2
and Msx2 were seen in periodontal fibroblasts of the tension side at 20 minutes
after mechanical stress. Expressions of Runx2 and Msx2 became stronger in
parallel with time, and at 24 hours after mechanical stress, the periodontal
fibroblasts, cementoblasts as well as osteoblasts showed strong expression. Moreover, ALP has
also demonstrated similar strong expression. The results strongly suggested that
Runx2 promoted differentiation of osteoblasts at
early stage and Msx2 worked as an activator of Runx2 function.