Immunohistochemical changes of Runx2 and Msx2 in mouse periodontal tissues elicited by orthodontic force

 

Takehiro Watanabe*,**, Rina Muraoka*,**, Takako Shimizu**, Keisuke Nakano*,***, Norimasa Okafuji*, Saburo Kurihara***, Kazuhiro Yamada*,**, Toshiyuki Kawakami*,***

Matsumoto Dental University Graduate School of Oral Medicine*

Matsumoto Dental University Hospital**

Matsumoto Dental University Institute for Oral Science***


  Early changes of Runx2 and Msx2 expressions were examined by
 immunohistochemistry in mouse periodontal ligament exposed to mechanical stress. Strong expressions of Runx2 and Msx2 were seen in periodontal fibroblasts of the tension side at 20 minutes after mechanical stress. Expressions of Runx2 and Msx2 became stronger in parallel with time, and at 24 hours after mechanical stress, the periodontal fibroblasts, cementoblasts as well as osteoblasts showed strong expression. Moreover, ALP has also demonstrated similar strong expression. The results strongly suggested that Runx2 promoted differentiation of osteoblasts at early stage and Msx2 worked as an activator of Runx2 function.