Asian Science Seminar ASS-4
Biological analysis of bone marrow stromal cells in vivo cell culture method
Mathieu Bernard Lefevre1, 2, Andrea Paola Rodriguez2, 3, Hidetsugu Tsujigiwa2, Hitoshi Nagatsuka2 and Noriyuki Nagai2
Oral Pathology and Medicine.
KUSA/A1 cells are immature mesenchymal stem cells isolated from bone marrow. These cells can differentiate to at least three types of mature cells like osteoblast, adipocyte and myoblast after treatment with 5-Azacytidine. Study showed that subcutaneous implantation of mature KUSA/A1 osteoblasts in diffusion chambers resulted in bone formation. It is well know that immature mesenchymal stem cells have better proliferation capabilities than mature cells. So, we hypothesized that the use of immature stem cells rather than mature cells could yield better results in tissue engineering due to the possibility of obtaining more cells to promote tissue repair.
this purpose, we have evaluated an appropriate medium to maintain KUSA/A1 cells (kindly
provided by Dr. Umezawa,
This study supports that immature KUSA/A1 cells show osteogenic potential activity in in vivo culture method.