OA-1

Induction of bone and cartilage by functionally graded-HAp / BMP-2 on rat skull periosteum

　　　　　　Jun Hino¹, Masaru Murata¹, Toshiyuki Akazawa², Mie Naraoka², Junichi Tazaki¹, and Makoto Arisue¹

¹Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido

²Department of Materials Technology, Hokkaido Industrial Research Institute

The objective of this study is to estimate the induction of bone and cartilage by absorbable hydroxyapatite block (fuctionally graded-hydroxyapatite:fg-HAp)/ rhBMP-2 (5μg) on rat skull periosteum, the absorption of fg-HAp and the observation of the periosteum histologically. As a result, body fluid permeated the inside of fg-HAp scaffold which were fragmented by degradation and absorption. The formation of hard tissue wasn’t found in the fg-HAp alone, while in the fg-HAp/BMP-2, the formation of bone and cartilage started in the outer layer of the implanted materials and the soft tissues including periosteum existed between the induced tissues and the skull bone. Therefore, it seems that periosteum has homeostasis as a boundary membrane of the hard tissue formation.